eckho Profile

eckho

Join Date:
2011-02-25

About
I am just an ordinary person who always wanted to work hard in order to realize the ideals I
My Social profiles

Blogs Owned

KUMPULAN BAHAN KULIAH KESEHATAN MASYARAKAT, KEPERAWATAN DAN KEBIDANAN, MAKALAH, ASKEP, ASKEB,.. SKRIPSI, KTI DL

Visit Follow

Other Tags: bahan kuliah kesehatan, makalah, skripsi, kti, askeb,askep

Latest Blog Posts

  • ASUHAN KEPERAWATAN DENGAN POST HERNIOTOMY AKIBAT HERNIA INGUINALIS LATERALASUHAN KEPERAWATAN DENGAN POST HERNIOTOMY AKIBAT HERNIA INGUINALISLATERALA.    Konsep Dasar1.     Definisia.      Hernia 1)     Hernia adalah menonjolnya s...
  • DIRECT ANTIGEN DETECTION FROM SPECIMENS (Appendix VI)
    on Aug 25, 2011 in VIROLOGY
    I. Introduction Immunofluorescent staining may be used to rapidly detect viral antigen directly in a clinical specimen. Smears prepared at the patient's bedside may be used, or a smear may be prepared in the laboratory from cellular material in a...
  • FECES/RECTAL
    on Aug 25, 2011 in VIROLOGY
    I. Introduction Faecal specimens may be submitted for the detection of viruses from patients with gastroenteritis, aseptic meningitis, viral encephalitis, or exanthem type rashes. Most cases of viral gastroenteritis are caused by viruses which do no...
  • DIRECT IMMUNOFLUORESCENT ANTIBODY (DFA) STAINING (Appendix V )
    on Aug 25, 2011 in VIROLOGY
    I. Introduction The DFA staining technique is used to detect viruses either directly in patient specimens or which have been isolated in shell vial or tube cultures. The method consists of a single staining step using a virus-specific antibody which...
  • TUBE CULTURE (not in routine use) or SHELL VIALS for CPE PROCEDURE (Appendix III)
    on Aug 25, 2011 in VIROLOGY
    I. Introduction Tube culture is the conventional method used by diagnostic virology laboratories for virus isolation. Since there is no universal cell line for recovery of all clinically significant viruses, a combination of cell types is used rou...
  • Appendix XVIII (Cont'd) QUALITY CONTROL OF MONOCLONAL ANTIBODIES
    on Aug 25, 2011 in VIROLOGY
    Reagent quality controls: These must be perform prior to patient testing to ensure each component of the reagent performs as expected. a. Check expiratory date then perform DFA, SimulFlour DFA or IFA accordingly. b. External QC slides (different m...
  • THROAT/NASOPHARYNGEAL/NASAL SWABS (SYMPTOMATIC)
    on Aug 25, 2011 in VIROLOGY
    I. Introduction These specimens are set up for routine isolation of respiratory viruses (Influenza A and B, Parainfluenza 1, 2, and 3, Respiratory Syncytial Virus, and Adenovirus). Direct antigen detection will be performed during the winter months...
  • SPESIMEN URINE
    on Aug 25, 2011 in VIROLOGY
    I. Introduction Urine is the specimen of choice for neonates suspected of congenital cytomegalovirus (CMV) infection. Other viruses recovered from urine that may be representative of a systemic infection include enteroviruses, rubella, measles, and...
  • GENITAL/PERI-ANAL/MOUTH/NOSE/SKIN LESIONS
    on Aug 25, 2011 in VIROLOGY
    I. Introduction Specimens from genital, perianal and oro-labial (mouth/nose) lesions will only be examined for herpes simplex virus (HSV) unless otherwise requested. Specimens from skin lesions will be examined for both herpes simplex virus (HSV) an...
  • BRONCHO-ALVEOLAR LAVAGE (BAL) - CMV SURVEILLANCE
    on Aug 25, 2011 in VIROLOGY
    I. Introduction Broncho-alveolar lavage specimens (BAL) for CMV surveillance collected on day 35 post-bone marrow transplant patients or routine screening of solid organ transplant patients are examined for CMV only.  http://bahankuliahkesehata...
  • Appendix XIII PRESERVATION OF CELL CULTURE MONOLAYERS & Appendix XIV QUALITY CONTROL OF CELL CULTURES USED FOR ROUTINE VIRUS ISOLATES
    on Aug 25, 2011 in VIROLOGY
    1. Aspirate the medium from the cell culture tubes to be preserved. 2. Add 8 mL buffered formaldehyde preservative medium to each tube. 3. Record the following information on the tube: - virus - number of days incubation - lab number 4. Store p...
  • ASSIGNMENT SHEET FOR VIROLOGY TERM PAPER Spring 2010
    on Aug 25, 2011
    Your assignment is to survey the primary literature in a well-focussed area of virology and to write a literature research paper (also known as a "term paper") of approximately 3000-4000 words reviewing the current state of knowledge in the...
  • SHELL VIAL PROCEDURE (Appendix II)
    on Aug 25, 2011 in VIROLOGY
    I. Introduction The shell vial method employs centrifugation of the patient specimen onto a cell monolayer contained in a vial. In general, the centrifugation step shortens the time to a positive culture result. Virus may be detected by direct flu...
  • Structure and Classification of Viruses
    on Aug 25, 2011 in VIROLOGY
    General Concepts Structure and Function Viruses are small obligate intracellular parasites, which by definition contain either a RNA or DNA genome surrounded by a protective, virus-coded protein coat. Viruses may be viewed as mobile genetic elemen...
  • CRYOPRESERVATION OF CELL CULTURES (Appendix X)
    on Aug 25, 2011 in VIROLOGY
    Generally research use only: 1. When a new bottle of cells to be frozen arrives, transfer 30 mL aliquots of cells into 125 cm2 flasks (about 3 flasks). 2. Incubate at 36oC until a complete monolayer is obtained (approximately 4 - 5 days). 3. Follo...
  • OCULAR SPECIMENS
    on Aug 25, 2011 in VIROLOGY
    I. Introduction Viral infections of the eye (conjunctivitis, corneal ulcers, etc) are usually due to herpes simplex virus (HSV), varicella-zoster virus (VZV) and adenoviruses. Ulcerative lesions are usually due to HSV and VZV. For vitreous fluid, th...
  • RECOVERY OF CRYOPRESERVED CELLS (Appendix XI)
    on Aug 25, 2011 in VIROLOGY
    Generally research use only: 1. Obtain the frozen cells from liquid nitrogen storage and immediately place the vial in a 37oC water bath WITH A PROTECTIVE COVER. ** Allow to thaw for 2 minutes (no more than 3 minutes). 2. Wipe the outside of the vi...
  • ETT/AUGER SUCTION (INFANTS)
    on Aug 25, 2011 in VIROLOGY
    I. Introduction Viral bronchitis, bronchiolitis and pneumonia are commonly caused by RSV or Parainfluenza in infants and young children. CMV pneumomitis may be seen in newborn/premature infants.    http://bahankuliahkesehatan.blog...
  • HEMADSORPTION OF TUBE CULTURE MONOLAYERS (NOT ROUTINELY DONE) Appendix VII
    on Aug 25, 2011 in VIROLOGY
    The hemadsorption (HAD) technique is used primarily to detect viruses that produce little or no cytopathic effect (CPE) in tube culture monolayers. Using guinea pig RBC, it is used to screen inoculated cell cultures for the presence of influenza, par...
  • THROAT/MOUTH/WASHINGS (PMH)
    on Aug 25, 2011 in VIROLOGY
    I. Introduction These specimens are routinely set up for herpes simplex virus only. Other viruses such as enteroviruses will be looked for only if specifically requested. Refer to Appendix XV (Virus Isolation and Identification) to ensure that the a...

Favorite Blogs (9)

1
Blogs
0
Followers
0
Following